Table 3.
Cell dimension measurements.
Cell dimensions in μm (±SEM) | ||||||
---|---|---|---|---|---|---|
Group | Treatment group | Height | Apical major width | Apical minor width | Basal major width | Basal minor width |
A | Morphology-control* | 27.2 ± 1.7 | 14.7 ± 0.5 | 8.7 ± 0.4 | 10.1 ± 0.4 | 7.1 ± 0.4 |
Particle-only group | ||||||
B | Particle/only-5 | 34.8 ± 0.9A | 15.6 ± 0.6 | 11.8 ± 0.5A | 13.3 ± 0.8A | 9.1 ± 0.4A |
C | Particle/only-60 | 37.2 ± 1.4A | 17.5 ± 0.9A | 12.2 ± 0.7A | 13.6 ± 0.3A | 9.9 ± 0.3A |
24 h Caco-2/macrophage group | ||||||
D | Macrophage/particle-5 | 27.5 ± 1.4B | 18.5 ± 0.8B | 13.4 ± 0.6B | 14.5 ± 0.5B | 10.6 ± 0.4B |
E | Macrophage/particle-60 | 23.9 ± 1.3C | 17.0 ± 0.5 | 14.0 ± 0.5C | 15.1 ± 0.5C | 11.2 ± 0.4C |
24 h macrophage-conditioned medium group | ||||||
F | Macrophage-conditioned medium/particle-5 | 32.0 ± 2.1D | 17.2 ± 0.5B | 10.8 ± 0.5D | 13.8 ± 0.5 | 9.0 ± 0.4D |
G | Macrophage-conditioned medium/particle-60 | 30.5 ± 1.1CE | 17.6 ± 0.6 | 10.6 ± 0.6E | 13.2 ± 0.3E | 9.1 ± 0.3E |
Mean values ± SEM for n = 27 samples are calculated from three independent experiments. *Data for group A are from Moyes et al. (2010). Statistical differences (P ≤ 0.05) between the data for one group and another are indicated by the use of the group letters defined in the first column. Apical widths, both major and minor for all treatment groups, are significantly larger than the equivalent basal dimensions (P = 0.09–0.0001).