Figure 2.

Linker-scanning mutagenesis of the –62+19 region of the cyclin D1 promoter. Various linker-scanning mutations of the cyclin D1 promoter from –62 to +19 were co-transfected into MCF7 cells together with a SV40 promoter-driven Renilla luciferase control plasmid. Mutated sequences are indicated by a solid black rectangle. Firefly luciferase activity for each construct was normalized to Renilla luciferase activity and is shown relative to that of the full-length wild-type promoter (–1745). The data are presented as means ± SEM for a minimum of two independent transfections done in triplicate unless otherwise indicated.