Figure 5.

The effect of GL020924 on cyclin D1 promoter reporter constructs in MCF7 cells. Various cyclin D1 promoter derivatives within the –310 to +155 context driving firefly luciferase in pGL3 basic were transfected into MCF7 cells. The constructs were wild-type (black), the –30 to –21 mutant (white), the 10 bp AT-rich promoter derivative (gray) and the 8 bp AT-rich promoter derivative (hatched). Four hours post-transfection, cells were incubated with or without GL020924 for 48 h, at which time promoter activities were assayed. Promoter activities were normalized relative to the co-transfected SV40 control driving Renilla luciferase and are expressed as a percentage of the untreated wild-type promoter construct. All samples were in triplicate; the error bars represent SEM for three separate experiments.