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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Urol. 2011 Jan;185(1):305–314. doi: 10.1016/j.juro.2010.09.008

Figure 3.

Figure 3

Aberrant ART877A activation of PSA transcription activity induced by DHT or cyproterone acetate (CA) on CV-1 cell cotransfection assay was inhibited by αE2 and βE2 via ERs. Cotransfection of PSA5.8-pGL3 reporter construct and ART877A expression vector ARL with or without ER expression vector ERα or ERβ in CV-1 cells was done as described. At 16 hours cells were treated with vehicle control, or various doses of single hormone (A and B) or hormone combination (C to F) for 48 hours. At experiment end luciferase activity was determined, normalized and shown in rlu. Data represent mean ± SEM of 4 to 8 individual samples in 2 to 4 independent experiments done in duplicate. Single asterisk indicates p <0.05 vs vehicle control. Double asterisks indicate p <0.01 vs vehicle control. Single pound sign indicates p <0.05 vs corresponding treatment in ARL transfected cells (A). Double pound signs indicate p <0.01 vs corresponding treatment in ARL transfected cells (A and B), 1 µM cyproterone acetate (C to E) and 50 nM DHT (F).