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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Urol. 2011 Jan;185(1):305–314. doi: 10.1016/j.juro.2010.09.008

Figure 5.

Figure 5

Aberrant ARL701H activation of PSA transcription induced by cortisol on CV-1 cell cotransfection assay was inhibited by αE2 and βE2 acting via ERs. Cotransfection of PSA5.8-pGL3 reporter construct and ARL701H expression vector with or without ER expression vector ERα or ERβ was done as described. At 16 hours cells were treated with vehicle control or 100 nM cortisol in presence or absence of 100 nM αE2 or βE2 for 48 hours. Luciferase activity was determined and expressed in rlu. Data represent mean ± SEM of 2 to 8 individual samples. Asterisks indicate p <0.01 vs vehicle control. Single pound sign indicates p <0.05 vs 100 nM cortisol in same transfection group. Double pound signs indicate p <0.01 vs 100 nM cortisol in same transfection group.