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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: Prostaglandins Other Lipid Mediat. 2010 Dec 15;94(1-2):34–43. doi: 10.1016/j.prostaglandins.2010.12.002

Table 1.

Specific hydrolysis activity for 2-OG and AEA in subcellular protein fractions of prostate carcinoma cells

Specific Hydrolysis Activity
(pmol/min/mg protein)
Cells Subcellular Fraction [3H3]2-OG [3H]AEA
PC-3 Cytosolic Fraction 37.43±6.51 0.79±3.43
Membrane Fraction 88.78±2.50 9.68±2.03
DU-145 Cytosolic Fraction 51.78±4.23 0.40±4.35
Membrane Fraction 162.97±6.09 21.39±1.60
LNCaP Cytosolic Fraction 22.90±2.99 1.31±3.10
Membrane Fraction 174.65±2.92 92.91±4.43

2-OG (2-AG) is the substrate for FAAH, MGL and other hydrolases ,whereas AEA is the substrate for FAAH (membrane bound enzyme) only.

The results are mean ± SEM (n = 6).

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