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. Author manuscript; available in PMC: 2011 Feb 15.
Published in final edited form as: J Neuroimmunol. 2009 Nov 10;218(1-2):83–93. doi: 10.1016/j.jneuroim.2009.10.006

Fig. 7.

Fig. 7

Reynolds infection induces significant hemolysis in brain tissue. Brain abscess homogenates from S. aureus strain RN6390- or Reynolds-infected animals (n=4 to 6 per group for each time point) as well as sterile bead injected mice (n=2 per group for each time point) were prepared at the indicated time points and analyzed for IgG (A) protein expression by ELISA and colorimetric hemoglobin (Hb) quantitation (B). Abscess-associated IgG levels were normalized to the amount of total protein recovered to correct for differences in tissue sampling size and reported as the mean values of IgG (picograms) per milligram of protein. Raw OD readings at 630 nm are used directly without any further normalization since all tissues were perfused prior to homogenization. The results represent the mean (±SEM) of three independent experiments. Significant differences between S. aureus strain RN6390- and Reynolds-infected mice are denoted with asterisks (*, p<0.05, **, p<0.001).