Fig. 8.
S. aureus regulates C3a induction in experimental brain abscess. Mice infected with either agarose-laden S. aureus strain RN6390- or S. aureus strain Reynolds-beads (104CFU) as described in Materials and methods, whereupon brain abscess protein extracts (40 μg per sample) were prepared at the indicated days post-infection and analyzed for C3 expression by Western blotting as described in Materials and methods. To compare with the baseline expression of C3 in the brain, naïve brain tissue was also included. Blots were stripped and reprobed with an antibody specific for actin to verify uniformity in gel loading. Results are presented from three individual animals per group and are representative of three independent experiments.