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. 2001 Feb 1;29(3):614–621. doi: 10.1093/nar/29.3.614

Figure 2.

Figure 2

Figure 2

AP sites can stimulate the formation of covalent Topo IV–DNA complexes. The DNA cleavage assay using Topo IV (24 nM) and the T440 DNA containing an AP site as DNA substrate (0.8 nM) was performed and the DNA products were analyzed as described in Materials and Methods. AP sites were located within the 4 base overhang generated by DNA scission (A) and immediately outside the two points of scission (B). Experiments were repeated three times and representative results are shown. The results of the quantification are shown in Table 1. Lane 0, T440(0); lane –2, T440(–2); lane –1, T440(–1); lane 1, T440(+1); lane 2, T440(+2); lane 3, T440(+3); lane 4, T440(+4); lane 5, T440(+5); lane 6, T440(+6). Size markers were 5′-end-labeled 64 and 31 nt oligos.