Figure 3.

Reversibility of formation of the AP site-induced covalent Topo IV–DNA complexes. An EDTA-mediated reversal assay was performed as described in Materials and Methods to assess reversibility of AP(–1)-, AP(+1)- and AP(+2)-induced covalent Topo IV–DNA complex formation. The Topo IV–norfloxacin–T440(0) ternary complex was used as a control. Reactions were terminated by addition of either SDS (open bars) or EDTA (closed bars). Experiments were repeated twice. The average values are shown and the error range was 4%. Abbreviations are as in the legend to Figure 2.