Figure 5.

AP site-induced covalent Topo IV–DNA complexes are sensitive to salt. Salt sensitivity of AP site-induced covalent Topo IV–DNA complexes was assessed as described in Materials and Methods. The relative amounts of cleaved DNA after a 5 min incubation in the absence (open bars) or presence (closed bars) of 0.5 M NaCl were measured. T440(–1), T440(+1) and T440(+2) were used as substrate and the Topo IV–norfloxacin–T440(0) ternary complex was used as a control. Experiments were repeated twice and the average values are shown. Error range was 6%. Abbreviations are as in the legend to Figure 2.