Table 1. Stimulation of Topo IV–catalyzed cleavage of T440 partial duplex DNAs containing an AP site by either the AP site or norfloxacin.
| DNA |
–Norfloxacin
(%)a |
+Norfloxacin
(%)b |
–Norfloxacin/+norfloxacin
(%)c |
| T440(0) | 6.5 | 78.3 | 8.3 |
| T440(–2) | 4.8 | 70.6 | 6.8 |
| T440(–1) | 56.2 | 74.5 | 75.4 |
| T440(+1) | 26.9 | 64.1 | 42.0 |
| T440(+2) | 40.2 | 86.3 | 46.6 |
| T440(+3) | 18.4 | 59.2 | 31.1 |
| T440(+4) | 19.6 | 77.2 | 25.3 |
| T440(+5) | 7.4 | 76.5 | 9.7 |
| T440(+6) | 3.0 | 82.4 | 3.6 |
Experiments were repeated three times.
aError range was ±2.8%.
bError range was ±5.9 %.
cAverage values were used for the calculation.