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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Eur J Immunol. 2010 Aug;40(8):2236–2247. doi: 10.1002/eji.200939759

Figure 3.

Figure 3

IL-23 Inhibits IL-12 Induced IFN-γ Production from Lymphocytes. Splenocytes from naïve WT B6 mice were cultured overnight in IL-2 supplemented media with or without 5ng/ml IL-12, IL-18, and 10 or 100ng/ml IL-23. Cells were stained for CD4 (A), NK1.1 (B), CD8 (C), and intracellular IFN-γ and were analyzed using flow cytometry. IFN-γ protein levels in culture supernatants were measured with ELISA (D). Two-way ANOVAs detected significant effects of IL-23 concentration. These data are representative of two independent experiments. An * indicates a difference from the 0ng/ml IL-23 culture condition, p ≤ 0.05. All data are expressed as the mean ± SEM (n = 2/group).