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. 2010 Dec 1;286(6):4199–4208. doi: 10.1074/jbc.M110.155093

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — The expression of C4ST-1 gene was suppressed in C2C12 cells expressing Wnt-3a. A, two cell lines of C2C12 cells expressing FLAG-tagged Wnt-3a proteins were established and named clone 1 (panels a and b) and clone 2 (panels c and d). C4ST-1 mRNA expression levels in these clones were examined by real-time PCR as described under “Experimental Procedures.” Panels a and c and panels b and d indicate the expression levels of Wnt-3a and C4ST-1, respectively. B, cellular and secreted FLAG-tagged Wnt-3a proteins of C2C12 cells expressing FLAG-tagged Wnt-3a, clones 1 and 2, were immunoprecipitated with anti-FLAG M2 affinity gel and then analyzed by immunoblotting using anti-FLAG antibody. Empty indicates C2C12 cells carrying a pIRES-neo3 empty vector. The total amount of Wnt-3a protein in clone 2 was expressed relative to that in clone 1 (right panel).