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. 2010 Dec 1;286(6):4199–4208. doi: 10.1074/jbc.M110.155093

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — C4ST-1 expression levels are positively correlated with E-disaccharide content and negatively correlated with Wnt-3a secretion. A, the amount of secreted Wnt-3a (top panel) and the content of E-disaccharide units in cellular CS chains (bottom panel) in L, L-Wnt-3a, and L-Wnt-3a-C4ST-1 transfectants (clone 2, clone 3, clone 4, and clone 11) were examined. Four L-Wnt-3a-C4ST-1 clones were ranked in order of increasing levels of C4ST-1 mRNA. Secretion of Wnt-3a into the medium was measured as described under “Experimental Procedures.” E-disaccharide content was determined based on HPLC analysis of CS chains isolated from each cell type as described under “Experimental Procedures.” B, Wnt-3a proteins in medium and cell lysates were analyzed using immunoblots probed with anti-Wnt-3a antibody. The amount of Wnt-3a protein in L and L-Wnt-3a-C4ST-1 clones was expressed relative to that in L-Wnt-3a cells. The average of the amount of Wnt-3a protein from two independent experiments is presented with S.D.