FIGURE 6.

Transient Wnt signaling has little effect on the accumulation of C4ST-1 mRNA. A and C, L cells were incubated in the presence of either 60 ng/ml rWnt-3a or 20 μm SB216763 for the indicated periods. Accumulation level of cytosolic β-catenin was measured as in Fig. 5A. The means from two independent experiments are plotted with the S.D. (error bars). B and D, total RNA was extracted from cells treated as described in A and C and subjected to quantitative real-time PCR. The expression level of C4ST-1 was calculated relative to that of GAPDH and normalized with the value at 0 h. E, human embryonic fibroblasts were treated with 60 ng/ml rWnt-3a for 6 h or 20 μm SB216763 for 12 h and then analyzed by immunoblotting. Accumulation level of cytosolic β-catenin was measured as in Fig. 5A. The means from two independent experiments are plotted with the S.D. (error bars).