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. 2010 Nov 29;286(6):4123–4132. doi: 10.1074/jbc.M110.171322

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FIGURE 3. — K_v2.1 is not likely N-glycosylated. Immunoblots of K_v2.1 and K_v1.4 ± N-glycosylation. Lysates isolated from transfected Pro5 cells. A, N-glycosylation site mutant and PNGase-treated lysates. K_v2.1 (left) and K_v1.4 (right). Lane 1, N-glycosylation site mutant; lane 2, PNGase-F treated; lane 3, untreated control. B, N-glycanase-treated. Lane 1, N-glycanase-treated; lane 2, untreated control. K_v2.1, 1.5–2.0 μg of protein loaded/lane. K_v1.4, 10 μg of protein loaded/lane.