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. 2010 Nov 29;286(6):4123–4132. doi: 10.1074/jbc.M110.171322

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FIGURE 7. — Click-iT chemistry confirmed the presence of sialylated O-glycans attached to K_v2.1, K_v4.2, and K_v4.3. A, immunoblots of K_v2.1, K_v4.2, and K_v4.3 Pro5 lysates. Lane 1, untreated whole cell lysates; lane 2, Ac₄ManNAz-labeled (sialic acid) samples; lane 3, Ac₄GalNAz-labeled (O-glycosylated) samples. Molecular weight markers are noted to the left of lane 1. B, control treatment in the absence of azido-modified sugars. Lane 1, untreated whole cell lysates. Lane 2, biotin-treated, streptavidin-precipitated samples. Top, K_v2.1. Middle, K_v4.2. Bottom, K_v4.3.