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. 2010 Dec 7;286(6):4912–4921. doi: 10.1074/jbc.M110.199729

FIGURE 2.

FIGURE 2.

ER stress preconditioning alleviated inducible nitric oxide synthase expression and inhibited NF-κB activation in HREC. A, HREC was pretreated with 0.1 μg/ml tunicamycin (TM) for 8 h followed by exposure to 10 ng/ml TNF-α for 4 or 24 h. inducible nitric oxide synthase (iNOS) expression were determined by Western blot analysis. B, nuclear translocation of NF-κB detected by immunocytochemistry in HREC after TNF-α treatment for 1 h. B, a–b, Control; B, c–d, TNF-α; B, e–f, TNF-α+TM. C, phosphorylation of NF-κB p65 subunit at Ser536 was determined by Western blot analysis. *, p < 0.05; **, p < 0.01 versus control; †, p < 0.05, ‡, p < 0.01 versus TNF-α. D and E, XBP1 splicing determined by RT-PCR (D) and Western blot analysis (E) in HREC after 0.1 μg/ml tunicamycin treatment for the indicated period. XBP1U, unspliced XBP1; XBP1S, spliced XBP1. *, p < 0.05; **, p < 0.01 versus control.