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. 2010 Nov 30;286(6):4819–4828. doi: 10.1074/jbc.M110.146860

FIGURE 2.

FIGURE 2.

Proteasome system and caspases do not mediate HDAC7 protein down-regulation. A, CGNs were treated for 24 h with HK or LK either in the absence or presence of 10 μm MG132 (MG). Phase contrast micrographs showing appearance of cultures at 24 h are shown in the left panel. Right panel shows viability of the cultures. The survival rates were normalized to HK. Data represent the mean ± S.D. from three independent experiments. * indicates p < 0.05 using the Student's t test compared with HK. B, CGNs were treated with HK or LK in the absence or presence of 10 μm MG132 for 6 h. Western blotting was performed to determine HDAC7 protein levels. Right panel shows quantification of HDAC7 protein expression. Relative levels were normalized to HK. Data represent the mean ± S.D. from three independent experiments. C, CGNs were treated with HK, LK, or LK medium containing 50 μm Z-VAD-fmk (Z-VAD) or 100 μm boc-Asp-fmk (BOC). HDAC7 protein levels were measured by Western blotting. Membranes were reprobed with cleaved caspase-3 antibody to ensure the effectiveness of Z-VAD-fmk and boc-Asp-fmk. HDAC7 protein levels were quantified by densitometric analysis with respect to tubulin. Relative levels were normalized to HK. Data represents the mean ± S.D. from three independent experiments.