FIGURE 3.

HDAC7 protects neurons from apoptosis. A, CGNs were transfected with GFP or FLAG-tagged HDAC7 and then switched to HK or LK medium for 24 h. Transfected cells were visualized by immunocytochemistry with GFP or FLAG antibody. Viability of transfected neurons was quantified by DAPI staining. The survival rates were normalized to GFP-transfected cultures treated with HK. Data represent the mean ± S.D. from five independent experiments. * indicates the value of p < 0.05 using the Student's t test (GFP LK versus HDAC7 LK). B, CGNs transfected with FLAG-HDAC7 were treated with HK or LK for 24 h after which immunocytochemical analysis was performed using FLAG antibody. Left panel shows typical localization pattern. Percentages of HDAC7 signal in cytoplasm (Cyt) or nuclei (Nuc) were counted. Right panel shows quantification from three independent experiments. * indicates the value of p < 0.05 using the Student's t test (HK cytoplasm versus LK Cyt, HK nuclei versus LK nuclei). C, cortical neurons were transfected with FLAG-HDAC7 or GFP for 8 h. The cells were then treated with 10 μm Aβ or vehicle (CTR). Two days later, cells were fixed and subjected to immunocytochemistry with GFP or FLAG antibody. Cell viability was quantified as described above. Result represents of mean ± S.D. from three independent experiments. * indicates the value of p < 0.05 using the Student's t test (GFP Aβ versus HDAC7 Aβ). D, cortical neurons were transfected with FLAG-HDAC7 or GFP. One day after transfection, cells were treated with 1 mm HCA or vehicle (CTR) for 16 h. Immunocytochemistry was performed to visualize transfected cells, and cell viability was quantified. Result represents of mean ± S.D. from three independent experiments. * indicates the value of p < 0.05 using the Student's t test (GFP HCA versus HDAC7 HCA).