FIGURE 6.

Effects of pharmacological inhibitors on HDAC7 neuroprotection. CGNs transfected with FLAG-HDAC7 were treated with different inhibitors 10 μm LY294002 (LY), 20 μm U0126, 50 μm PD98059 (PD), 10 μm Gö6983 (Gö), 50 μm KN62 (KN), or 5 μm AKT inhibitor X (AKTX) in either HK or LK for 24 h. HK or LK medium containing DMSO was used as control (CTR). Viability was determined by immunocytochemistry and DAPI staining and compared with GFP-transfected cultures treated with HK. Data represent the mean ± S.D. from three independent experiments.