FIGURE 6.

IP3KB-mediated modulation of carbachol-induced Ca²⁺ signals in HeLa cells. HeLa cells were either mock transfected (A) or transfected with expression constructs for wild type (B) or mutant IP3KB with reduced enzymatic activity (C). Wild type and mutant IP3KB were fused with EGFP. Approximately 24 h post-transfection, cells were loaded with Fura-2/AM and subjected to single-cell Ca²⁺ imaging. 1.5 min after start of the recording, carbachol was added to the bath solution to a final concentration of 100 μm. Gray lines show single cell ratio tracings; black lines indicate the calculated means.