FIGURE 7.

Sequence motifs mediating nucleocytoplasmic shuttling and actin binding are located in the multitargeting domain. H1299 and HeLa cells transiently expressing EGFP fusion proteins were fixed, and the nuclear/cytoplasmic ratio was determined. Wild type IP3KB (WT), a mutant form lacking the multitargeting domain (ΔMTD), and the isolated multi-targeting domain fused with EGFP (MTD) were used. A final concentration of 20 nm LMB and an incubation time of 6 h were used (+LMB). Further details can be found in the legend to Fig. 2. For detailed information about the constructs, see Table 1.