TABLE 1.
Fusion genes and fusion gene derivates
Full-length cDNA and different fragments of HsIP3KB were cloned into the EGFP fusion gene expression vector pEGFP-N1. Subsequently, the full-length cDNA was altered by deletion and substitution mutagenesis.
| Construct | Description |
|---|---|
| HsIP3KB/EGFP | Full-length/WT |
| ΔNES | Δ 138–143 |
| mNES | 138LQNVQV→AQNAQA |
| NES/EGFP | 132–145 |
| EGFP/NES | 132–145 |
| mNLS-1 | 128KRKLR→QRKLR |
| mNLS-2 | 128KRKLR→KQKLR |
| mNLS-3 | 128KRKLR→KRQLR |
| mNLS-4 | 128KRKLR→KRKLQ |
| mNLS-5 | 128KRKLR→QQQLR |
| NLS/EGFP | 126–134 (126EAKRKLRIL) |
| mFEA-1 | 154IQAQS→PQAQP |
| mFEA-2 | 150FE→QA |
| ΔMTD | Δ103–165 |
| MTD/EGFP | 104–165 |