FIGURE 1.

Design and characteristics of the SeVdp vectors. A, genome structure of the Sendai virus and an SeVdp vector is shown. Exogenous genes installed on the SeVdp vector are indicated as A–D. B, genome structure of SeVdp(KR/Bsr/EGFP/KO) is shown. C, expression of the fluorescent marker genes installed on the SeVdp vector is shown. LLCMK₂ cells were infected with SeVdp(KR/Bsr/EGFP/KO) at a multiplicity of infection of 0.1, selected with blasticidin S (5 μg/ml), and examined by fluorescence microscopy with dye-specific filters. D, stability of gene expression induced by the SeVdp vector is shown. LLCMK₂ cells were infected with SeVdp(KR/Bsr/EGFP/KO) and selected with blasticidin S as described in C. The cells were then cultured for the indicated period in the absence of blasticidin S. The ratio of SeV NP antigen-positive cells in the total cells was determined by fluorescence microscopy, as described under “Experimental Procedures.”