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. 2010 Dec 2;286(6):4620–4631. doi: 10.1074/jbc.M110.195313

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 4. — Cdc34 is required degradation of processed Stp1 but not processed Stp2. A, a cdc34-2 temperature-sensitive mutation inhibits degradation of Stp1ΔN-HA. Wild-type (BY4741) and nine isogenic ubcΔ mutant strains, and a cdc34-2 mutant strain (MGG15) expressing STP1ΔN-HA (pZL1635) were grown in SD medium + leucine and stability of Stp1ΔN-HA was determined. B, mutations in CDC34 and CDC53 stabilize natively processed Stp1-HA (Stp1C-HA). Wild-type (ZLY044), cdc34-2 (MGG15), and cdc53-1 (MGG12) mutant strains expressing STP1-HA were analyzed for Stp1C-HA stability. C, a cdc34-2 mutation fails to increase the stability of Stp2C. cdc34-2 (MGG15) mutant cells expressing Stp2-HA were grown in SD medium supplemented with leucine and stability of Stp2C was determined in cells grown at 26 and 37 °C.