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. 2010 Dec 2;286(6):4620–4631. doi: 10.1074/jbc.M110.195313

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — Differential localization of Stp1 and Stp2. A, fluorescence microscopic analysis of intracellular localization of Stp1-GFP and Stp2-GFP. stp1Δ mutant cells (RBY881) expressing STP1-GFP (pZL2813) and stp2Δ mutant cells (RBY903) expressing STP2-GFP (pZL2849) were grown in SD medium ± leucine and GFP fluorescence images were captured as described under “Experimental Procedures.” B, plasma membrane localization of Stp1-GFP does not require Ssy1, Ptr3, Ssy5, or Grr1. Wild-type (ZLY044) and isogenic ssy1Δ (ZLY1915), ptr3Δ (ZLY1917), ssy5Δ (ZLY1939), and grr1Δ (ZLY1942) mutant strains expressing STP1-GFP were grown in SD medium and GFP fluorescence images were captured.