TABLE 2.
Plasmids used in this study
| Plasmid | Description | Source/Ref. |
|---|---|---|
| pZL1834 | pRS416-STP1-HA, expressing Stp1 with 3× HA tagged at the C terminus | 11 |
| pZL2508 | pRS416-STP2-HA, expressing Stp2 with 3× HA tagged at the C terminus | This study |
| pZL2841 | pRS426-STP1-HA, expressing Stp1with 3× HA tagged at the C terminus | This study |
| pZL2543 | pRS416-GAL1-STP1-HA3-HIS12, expressing Stp1 under control of GAL1 promoter with both a 3× HA and a 12× His tag at the C-terminal end | This study |
| pZL2540 | pRS416-GAL1-STP2-HA3-HIS12, expressing Stp2 under control of GAL1 promoter with both a 3× HA and a 12× His tag at the C-terminal end | This study |
| pZL1553 | pRS415-GAL1-GRR1-myc3, expressing Grr1 under control of GAL1 promoter with 3× myc tag at the C-terminal end | 48 |
| pZL1635 | pRS416-STP1ΔN-HA3, expressing a N-terminal truncation mutant of Stp1 missing the first 97 residues under the native promoter with a 3× HA tag at the C terminus | This study |
| pZL1637 | pRS416-STP2ΔN-HA3, expressing an N-terminal truncation mutant of Stp2 missing the first 105 residues under the native promoter with a 3× HA tag at the C terminus | This study |
| pZL2813 | pRS416-STP1-GFP, expressing C-terminal GFP-tagged Stp1 | This study |
| pZL2849 | pRS416-STP2-GFP, expressing C-terminal GFP-tagged Stp2 | This study |
| pZL2615 | pRS416-STP1(1–125)-GFP, expressing the Stp1(1–125)-GFP fusion | This study |
| pZL2862 | pRS416-STP1(1–159)-GFP, expressing the Stp1(1–159)-GFP fusion | This study |
| pZL2865 | pRS416-STP1(1–273)-GFP, expressing the Stp1(1–273)-GFP fusion | This study |
| pZL2667 | pRS416-STP1(1–299)-GFP, expressing the Stp1(1–299)-GFP fusion | This study |
| pZL2867 | pRS416-STP1(1–331)-GFP, expressing the Stp1(1–331)-GFP fusion | This study |
| pZL2869 | pRS416-STP1(1–376)-GFP, expressing the Stp1(1–376)-GFP fusion | This study |
| pZL2661 | pRS416-STP1(267–519)-GFP, expressing the Stp1(267–519)-GFP fusion | This study |
| pZL2669 | pRS416-STP1(291–519)-GFP, expressing the Stp1(291–519)-GFP fusion | This study |
| pZL2671 | pRS416-STP1(299–519)-GFP, expressing the Stp1(299–519)-GFP fusion | This study |
| pZL2663 | pRS416-STP1(331–519)-GFP, expressing the Stp1(331–519)-GFP fusion | This study |
| pZL2677 | pRS416-STP1(267–376)-GFP, expressing the Stp1(267–376)-GFP fusion | This study |
| pZL2665 | pRS416-STP1(267–331)-GFP, expressing the Stp1(267–331)-GFP fusion | This study |
| pZL2879 | pRS416-STP1(Δ330–336)-GFP, expressing a GFP-tagged Stp1(Δ330–336) truncation construct with residues 330–336 of Stp1 deleted | This study |