Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Hepatocyte furin is the major PCSK9 cleaving enzyme at Arg218. Primary hepatocytes lacking both PC5/6 and furin were transiently cotransfected with plasmids expressing no protein (V), V5-tagged human PCSK9 (WT, F216L, R218S, and RRRR218EL) and no protein (V), furin (Fur), or PC5/6A. After 48 h, conditioned media were directly analyzed by Western blotting using a V5 antibody. Note the presence of a faster migrating PCSK9 species (PCSK9-ΔNx).
