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. 2010 Oct 21;286(6):4090–4097. doi: 10.1074/jbc.M110.173096

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FIGURE 4. — Hypoxia reduces BRE activity in the hepcidin promoter through HIF and TMPRSS6. A, HJV-Hep3B cells were co-transfected with one of the luciferase vectors (wtBRE, mutBRE) in combination with control siRNA or HJV siRNA. Levels of luciferase signal were then measured after 48 h (*, p < 0.001 using Bonferroni's post-test analysis of one-way ANOVA). HJV-Hep3B cells were transfected with one of the luciferase vectors wtBRE (B), mutBRE (C), or CA9-HRE (D) in combination with control siRNA, TMPRSS6 siRNA or combined HIF-1α and -2α siRNA. Levels of the luciferase signal were then measured after 48 h of normoxia or hypoxia (0.5% O₂) (*, p < 0.001 using Bonferroni's post-test analysis of one-way ANOVA). Luciferase signal was normalized to β-galactosidase signal to correct for transfection efficiency.