FIGURE 1.

G6 inhibits Jak2-V617F-dependent HEL cell proliferation, in vitro. A, HEL cells were treated with increasing doses of G6 for 72 h, and the number of viable cells was determined. B, HEL cells were treated 25 μm G6 for 0, 6, 24, 48, and 72 h and cell numbers were determined. C, HEL cells were treated with 25 μm of G6 for 0, 6, 12, 24, 48, and 72 h. At the end of each time point, the cells were placed in medium lacking inhibitor for an additional 72 h. The number of viable cells was then determined. Shown are mean ± S.E. for three independent experiments, each run in triplicate.