TABLE 1.
Mass spectrometry results showing plasma and tissue concentrations of G6 at euthanasia
The standard curve was quadratic (1/×2) r = 0.9902 ranging from 0.005–5.00 μg/ml.
| Animal ID | Plasma | Bone Marrow | Spleen |
|---|---|---|---|
| μg/mla | mg/g of tissueb | mg/g of tissueb | |
| Vehicle-treatedc | |||
| 1 | No Peak | No Peak | No Peak |
| 2 | <0 | No Peak | No Peak |
| 3 | No Peak | No Peak | No Peak |
| 4 | No Peak | No Peak | No Peak |
| 5 | No Peak | No Peak | No Peak |
| 6 | No Peak | No Peak | No Peak |
| G6-treated | |||
| 1 | 0.010 | 0.249 | 0.402 |
| 2 | 2.710 | 0.106 | 0.063 |
| 3 | 0.024 | 1.490 | 0.336 |
| 4 | <0 | 0.057 | 0.205 |
| 5 | 0.023 | 0.041 | 0.203 |
| 6 | 2.700 | 0.025 | 0.834 |
a Average of replicate injections.
b Calculations based on average of four replicate injections back-calculated using analyte concentrations (mg/ml) divided by prepared tissue concentration (g/ml) resulting in mg/g of tissue concentrations.
c <0 indicates peak quantitates were below the 0 value of the standard curve. No Peak indicates no peak was observed in raw chromatography.