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. 2010 Dec 3;286(6):4882–4891. doi: 10.1074/jbc.M110.147512

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 6. — LePRK2 but not mLePRK2 induced a stable actin network at the pollen tube tip. eGFP (A), LePRK2-eGFP (B), or mLePRK2-eGFP (C) were transiently co-expressed with mRFP-mTalin in tobacco pollen and visualized using confocal microscopy (RFP, left panels; eGFP, right panels). Scale bar represents 100 μm. Medial sections of the pollen tube are shown. Far right panels showed projection of the whole pollen tube with merged fluorescent emissions (eGFP+RFP). D, effect of latrunculin B (Lat B) on pollen tube length and tip width of pollen tubes overexpressing eGFP, LePRK2-eGFP, and mLePRK2-eGFP. *, p < 0.05; **, p < 0.01; and ***, p < 0.001.