FIGURE 10.

CerS6 knockdown protected OLs against NGF-induced apoptosis. A, OLs were exposed to 1 mm NGF with/without TrkA receptor inhibitor, 50 μm K252a, or 1 μg/ml of anti-NGF receptor antibody, and cell death was measured 24 h later. B, OLs were transfected with 1 nm CerS6-specific siRNA or 1 nm CerS5-specific siRNA or control siRNA (CSR) and cultured for 24 h. Then, cells were treated with 1 mm NGF, 50 μm K252a, and cell survival was measured 24 h later. Data are mean ± S.E., *, p < 0.05, n = 12. C, OLs were transfected with 1 nm CerS6-specific siRNA control siRNA (CSR) and cultured for 24 h. Then, cells were treated with 1 mm NGF, 50 μm K252a, and ceramide species content was measured 24 h later. Data are mean ± S.E., *, p < 0.05, n = 12.