FIGURE 11.

Glutamate- or NGF-induced OL apoptosis involves disturbance of mitochondrial Ca²⁺ and calpain activation. A, OLs were exposed to glutamate with/without the mitochondrial Ca²⁺-uniporter inhibitor, 1 μm RU360, or calpain inhibitors, 1 μm calpeptin or 1 μm PD150606. Cell death was assessed 24 h later. Data are mean ± S.E., *, p < 0.05, n = 16. B, OLs were exposed to 1 mm NGF plus 20 μm K252a with/without 1 μm RU360 or 1 μm calpeptin or 1 μm E64d or 1 μm E64c, and cell death was measured 24 h later. Data are mean ± S.E., *, p < 0.05, n = 12. C, OLs were transfected with 1 nm CerS6-specific siRNA or control siRNA (CSR) and cultured for 24 h. Then, cells were treated with glutamate for 24 h and calpain activity was measured. Data are mean ± S.E., *, p < 0.05, n = 6.