FIGURE 4.

Ceramide synthases are differentially expressed during brain development. Ceramide synthase protein expression was analyzed in brain tissue (A) or in mitochondria (C) by Western blot using specific antibodies. Membranes were stripped and probed with anti-β-actin antibody (brain) or anti-voltage-dependent anion channel (VDAC) antibody (mitochondria) to confirm equal loading of samples. B and D, quantification of the ceramide synthases expression in brain (B) or mitochondria (D). Data are mean ± S.E. (from 4 independent experiments).