FIGURE 6.

CerS6 is associated with ANT, whereas CerS2 is associated with protein import complex. Mitochondria were purified from the brain of an adult rat. Mitochondria lysate (10 μg) was loaded into the lane (Input). Complexing with each CerS was detected by immunoprecipitation using antibodies against Tom20, a receptor of the outer mitochondrial membrane protein import complex (A); voltage-dependent anion channel (VDAC), outer mitochondrial membrane component of MPTP (B); cyclophilin D (CyD), matrix protein-regulator of MPTP (C); or ANT, the inner mitochondrial membrane component of MPTP (D). As a control, the same immunoprecipitation procedure was performed except for primary antibody application (IgG).