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AMOTL1 and AMOTL2 regulate YAP1 subcellular localization. A, the localization of endogenous YAP1 was revealed by anti-YAP1 immunostaining in cells with or without AMOTL1 or AMOTL2 overexpression. B–D, HeLa cells were transfected with plasmids encoding Myc-tagged AMOTL1 or AMOTL2 with plasmids encoding SFB-tagged wild-type YAP1 (B), YAP1 mutant with deletion of its first WW domain (C), or YAP1 S127A mutant (D). Immunostaining was conducted using antibodies as indicated. M, merged.
