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Constitutively active TLR13 (CD4-TLR13) activates both NF-κB and IFN-β. HEK293 cells (A and B) and MEFs (C and D) transiently transfected with expression vectors for CD4-TLR13, CD4-TLR4, or empty expression vector (control) together with an NF-κB or IFN-β luciferase reporter are shown. Luciferase activity was measured with a luminometer after 24 h transfection. Asterisk, p < 0.05. The data are representative of three similar experiments.
