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. 2001 Feb 1;29(3):809–817. doi: 10.1093/nar/29.3.809

Table 1. Oligonucleotide primer sequences.

Oligo
5′-End
Sequence
Product (bp)
33.3 UP 33293 AACAAAAGCAAAACCAAA 431
33.3 LP 33723 GTATGTAGGCACCCGATG  
33.3 IP 33368 TCCTGCCATGTTAAGTG  
33.3 MP 33293 AACAAAAGCAAAACCAAACCAAAGAAAAA  
40.9 UP 40944 GCAAGCAATACAAATAAT 156
40.9 LP 41099 ACCACAAACACAAACAGG  
40.9 IP 41016 TATATGAGCCACAAAGGG  
40.9 MP 41099 ACCACAAACACAAACAGGCACTTCCCTCAATATAAAC  
54.8 UP 54806 CTGAGGAGAAGACTGCTGT 419
54.8 LP 55224 CTGGACTCACCCTGAAGTT  
54.8 IP 54977 TCTCTGTCCCTTGGGCTG  
55.5 UP 55535 TAATCCTTTTGTCTCTCC 195
55.5 LP 55729 CCCACCTCCAGTGTAACT  
55.5 IP 55568 AGGCTCCAACTCAAAGAT  
55.5 MP 55729 CCCACCTCCAGTGTAACTTATTCACTTTCCTTTCC  
62 UP 62005 CAGGTACGGCTGTCATCACTT 210
62 LP 62214 CAGGGCAGTAACGGCAGA  
62 MP 62214 CAGGGCAGTAACGGCAGAATGGTGTCTGTT  
72 UPa 71918 TTGACAAACCTGAGAGAA 536
72 LPa 72453 TGATGGCTAGTGATGATG  
72 IP 72132 GGCATGGGCAAGGACTTC  
72 UPb 72319 CCAGAATCTACAATGAACTC 135
72 LPb 72453 TGATGGCTAGTGATGATG  
72 MP 72453 TGATGGCTAGTGATGATGTGATTACATAAATGTCTT  

The upper (UP, rightward) and lower (LP, leftward) primers used for PCR mapping and competitive PCR are shown, as well as the internal probes (IP) for PCR mapping and the mutagenic primers (MP) for competitor construction. The 5′-end position of each oligonucleotide is given along with the size of each STS PCR product in base pairs.

aUsed for PCR mapping.

bUsed for competitive PCR.