Table 1. Oligonucleotide primer sequences.
Oligo |
5′-End |
Sequence |
Product (bp) |
33.3 UP | 33293 | AACAAAAGCAAAACCAAA | 431 |
33.3 LP | 33723 | GTATGTAGGCACCCGATG | |
33.3 IP | 33368 | TCCTGCCATGTTAAGTG | |
33.3 MP | 33293 | AACAAAAGCAAAACCAAACCAAAGAAAAA | |
40.9 UP | 40944 | GCAAGCAATACAAATAAT | 156 |
40.9 LP | 41099 | ACCACAAACACAAACAGG | |
40.9 IP | 41016 | TATATGAGCCACAAAGGG | |
40.9 MP | 41099 | ACCACAAACACAAACAGGCACTTCCCTCAATATAAAC | |
54.8 UP | 54806 | CTGAGGAGAAGACTGCTGT | 419 |
54.8 LP | 55224 | CTGGACTCACCCTGAAGTT | |
54.8 IP | 54977 | TCTCTGTCCCTTGGGCTG | |
55.5 UP | 55535 | TAATCCTTTTGTCTCTCC | 195 |
55.5 LP | 55729 | CCCACCTCCAGTGTAACT | |
55.5 IP | 55568 | AGGCTCCAACTCAAAGAT | |
55.5 MP | 55729 | CCCACCTCCAGTGTAACTTATTCACTTTCCTTTCC | |
62 UP | 62005 | CAGGTACGGCTGTCATCACTT | 210 |
62 LP | 62214 | CAGGGCAGTAACGGCAGA | |
62 MP | 62214 | CAGGGCAGTAACGGCAGAATGGTGTCTGTT | |
72 UPa | 71918 | TTGACAAACCTGAGAGAA | 536 |
72 LPa | 72453 | TGATGGCTAGTGATGATG | |
72 IP | 72132 | GGCATGGGCAAGGACTTC | |
72 UPb | 72319 | CCAGAATCTACAATGAACTC | 135 |
72 LPb | 72453 | TGATGGCTAGTGATGATG | |
72 MP | 72453 | TGATGGCTAGTGATGATGTGATTACATAAATGTCTT |
The upper (UP, rightward) and lower (LP, leftward) primers used for PCR mapping and competitive PCR are shown, as well as the internal probes (IP) for PCR mapping and the mutagenic primers (MP) for competitor construction. The 5′-end position of each oligonucleotide is given along with the size of each STS PCR product in base pairs.
aUsed for PCR mapping.
bUsed for competitive PCR.