TABLE 1.

Binding affinities of epibatidine, nicotine, and acetylcholine for wild type Ac-AChBP, Y53C AChBP, and the MMTS- and MTSET⁺-modified Y53C proteins

Student's t-test was used to determine significance levels.

	Kd [3H]EB	Ki nicotine	Ki acetylcholine
	nm	μm	μm
wt Ac-AChBP	8.47 ± 1.93	0.77 ± 0.06	64.4 ± 8.6
Y53C	26.76 ± 4.02a	2.78 ± 0.47b	60.65 ± 2.53
Y53C-MMTS	19.11 ± 3.77a	3.05 ± 0.28b	98.3 ± 0.8a
Y53C-MTSET+	NBc	NB	NB

^a Different from wt p < 0.05.

^b Different from wt p < 0.001.

^c NB, no binding.