Transcriptional pattern analysis and RNAi of OfHEX1. A, transcriptional pattern of OfHEX1 at different developmental stages of O. furnacalis. 4L, fourth instar larvae; 5L1, fifth instar larvae (1st day); 5L2, fifth instar larvae (2nd day); 5L3, fifth instar larvae (3rd day); 5L4, fifth instar larvae (4th day); 5L5, fifth instar larvae (5th day); PP, prepupae; P1, pupae (early); P2, pupae (medium); P3, pupae (late); A, adults. B, transcriptional pattern of OfHEX1 in different tissues of O. furnacalis. C, normal larva/pupa and three abnormal phenotypes of dsOfHEX1-injected insects. D, effect of RNAi on the transcriptional levels of OfHEX1. Data are the mean ± S.E. (error bars) from three independent experiments.