FIGURE 2.

Overall structure of OfHex1. A, overall structures of dimeric OfHex1. Domain I and domain II are shown in light blue and wheat, respectively. Four unique structural elements in OfHex1 are shown in different colors. The N-terminal and C-terminal additional structures involved in dimerization are shown in orange and red, respectively. L_314–335 and L_478–496 are shown in pink and green, respectively. B, comparison of L_314–335 and L_478–496 in the OfHex1-TMG-chitotriomycin complex (blue) with corresponding regions in the SmCHB-chitobiose complex (1QBB) (green), SpHex-NGT complex (1HP5) (pink), and human HexB-NGT complex (1NP0) (yellow). The conserved residues Val³²⁷ (Val⁴⁹³ in SmCHB and Val²⁷⁶ in SpHex) and Trp⁴⁹⁰ (Trp⁶⁸⁵ of SmCHB and Trp⁴⁰⁸ of SpHex) are shown as sticks.