TABLE 2.

Kinetic parameters of WT and mutants of OfHex1

Data are the means ± S.D. of three determinations.

	Substrate	Km	kcat	kcat/Km
		mm	s−1	s−1mm−1
WT	4MU-β-GlcNAc	0.107 ± 0.009	434.7 ± 13.3	4063
V327G	4MU-β-GlcNAc	0.070 ± 0.003	189 ± 4.9	2700
W490A	4MU-β-GlcNAc	0.100 ± 0.006	167.6 ± 4.1	1676
WT	(GlcNAc)2	0.148 ± 0.009	507.4 ± 32.5	3428
V327G	(GlcNAc)2	0.126 ± 0.008	337 ± 27	2675
W490A	(GlcNAc)2	1.875 ± 0.042	449.6 ± 19.8	239.8
W448A	4MU-β-GlcNAc	0.223 ± 0.011	0.469 ± 0.032	2.103
W448F	4MU-β-GlcNAc	0.149 ± 0.008	0.567 ± 0.044	3.805
H433A	4MU-β-GlcNAc	0.095 ± 0.004	0.313 ± 0.018	3.295
E328A	4MU-β-GlcNAc	0.096 ± 0.004	356 ± 17.3	3708
E328Q	4MU-β-GlcNAc	0.105 ± 0.005	349 ± 15.4	3324