FIGURE 2.

Role of Nej1 in the binding of yKu to an in vivo DSB: effect of genetic inactivation of NEJ1 on the recruitment of Rad51 to an in vivo DSB and sensitivity to phleomycin. The kinetics of recruitment of yKu to a site-specific DSB in SLY1A (WT), SLY1A ΔLIF1 (lif1), and SLY1A ΔNEJ1 (nej1) strains were measured by chromatin immunoprecipitation with (A) and without (B) formaldehyde cross-linking. C, kinetics of recruitment of Rad51 to a site-specific DSB in SLY1A (WT), SLY1A ΔLIF1 (lif1), SLY1A ΔyKU70 (yku), and SLY1A ΔNEJ1 (nej1) strains. D, growth of wild-type SLY1A (WT) and derivatives with the indicated gene deletions was compared on Yeast Extract Peptone-glucose plates containing increasing concentrations of phleomycin by spotting dilutions of the cultures onto plates.