FIGURE 5.

Interaction of yKu, Nej1, and Dnl4-Lif1 with DNA visualized by surface plasmon resonance. A, representative sensorgram generated by sequential injection of Ku (1 nm), Nej1 (1 nm), and Dnl4-Lif1 (10 nm) onto a sensor chip coated with 669-bp blunt-ended duplex DNA. B, representative sensorgrams showing the profile of binding of Nej1 (1.25 nm) to the DNA chip that had either been preloaded with yKu (2 nm; blue line, Ku) or not (black line, No protein). C, representative sensorgrams showing the profile of binding of Dnl4-Lif1 (10 nm) to the DNA chip (black line, No protein) and the effect of preloading of the DNA chip with yKu (1 nm; blue line, Ku) or yKu followed by Nej1 (1 nm yKu and 1 nm Nej1; red line, Ku → Nej1). D, representative sensorgrams showing protein binding to the DNA chip preloaded with yKu followed by Nej1 alone (1 nm; blue line, Ku → Nej1), Dnl4-Lif1 alone (10 nm; black line, Ku → Dnl4), and a mixture of Nej1 and Dnl4-Lif1 (1 nm Nej1 and 10 nm Dnl4-Lif1, red line, Ku → Nej1 + Dnl4). RU, resonance units.