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. 2010 Dec 13;286(6):4931–4940. doi: 10.1074/jbc.M110.195024

FIGURE 7.

FIGURE 7.

Effect of Nej1 on DNA joining by Dnl4-Lif1 under single- and multiple-turnover reaction conditions. A, ligation of cohesive-ended duplex DNA (lanes 1–6; DSB 5′ 4nt; 1 pmol; open squares) and nicked DNA (lanes 7–12; Nick; 1 pmol; open circles) in the absence of ATP. B, ligation of cohesive-ended duplex DNA (lanes 1–6; DSB 5′ 4nt; 10 pmol; filled squares) and nicked DNA (lanes 7–12; Nick; 10 pmol; filled circles) in the presence of ATP. The reaction contained Dnl4-Lif1 (1 pmol) alone (lanes 1 and 7) and increasing amounts of Nej1 (lanes 2 and 8, 0.5 pmol; lanes 3 and 9, 1 pmol; lanes 4 and 10, 1.5 pmol; lanes 5 and 11, 2 pmol; lanes 6 and 12, 3 pmol). C, kinetics of joining of cohesive-ended duplex DNA (10 pmol) by Dnl4-Lif1 (1 pmol) in the absence (No Nej1; open squares) or presence (With Nej1; 2 pmol; filled squares) of Nej1 in reactions containing ATP (1 mm). The results of three independent experiments are shown graphically. Error bars indicate mean ± S.D.

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