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. Author manuscript; available in PMC: 2012 Jan 10.
Published in final edited form as: Vaccine. 2010 Nov 9;29(3):476–486. doi: 10.1016/j.vaccine.2010.10.072

Fig. 1.

Fig. 1

Construction of Lmdd-BdopSIVgag and analysis of SIV gag expression by Lmdd-BdopSIVgag. The recombinant Listeria monocytogenes (Lm) vector Lmdd-BdopSIVgag was obtained by allelic exchange between the Lm dal dat vector and pKSV-7 carrying a Bacillus subtilis dal gene and Lm-codon-optimized SIV gag (A). Culture supernatants from control Lmdd-Bdop (lanes 1, 2) and from gag-expressing Lmdd-BdopSIVgag (lanes 3–6) were analyzed by Western blot. SIV Gag was detected using the anti-SIV Gag monoclonal antibody 2F12 (AIDS Research & Reference Reagent Program, NIH). Cultures contained approximately 7 ng/ml p27 antigen as determined by ELISA. The multiple bands reflect proteolytic processing of Gag within the Lm and following secretion into the medium.