Table 1.

Effect of mitochondrial metabolism inhibitors on the uptake of paromomycin

Treatment	Inhibitor concentration	Mean % of control accumulation
Control−25°C	—	100.0
4°C	----	75.5 ± 5.5
Cyanide	1 mM	71.9 ± 10*
Azide	5 mM	81.4 ± 1.5*
Oligomycin	15 µM	115.9 ± 20
Valinomycin	10 µM	98.9 ± 6.4
CCCP	10 µM	88.1 ± 8.9
Ouabain	1mM	69.6 ± 8.9*
Azide + 2-deoxy-D-glucose	0.1% + 50 mM	66.8 ± 8.0*

Cells were pretreated with inhibitors at 25 °C for 10 min and then incubated with 100 µM paromomycin for 10 min in PBSG. All values are given as a percentage of the wild-type control. Results are means ± S.D. of at least two independent experiments.

^*is statistically different at P<0.05, when compared to the values obtained for control 25°C.